Purine-Requiring Auxotrophs of Coprinus lagopus (sensu Buller)
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چکیده
منابع مشابه
Factors affecting the level and activity of pyruvate kinase from Coprinus lagopus sensu Buller.
The specific activity of Coprinus lagopus pyruvate kinase is high under conditions of glycolysis but low under conditions of gluconeogenesis. Only a single form of the enzyme was detectable, the properties of which resemble in a number of respects those of the allosteric forms of pyruvate kinase. These properties include co-operative interactions with phosphoenolpyruvate (PEP), ATP and fructose...
متن کاملPurine requiring strains of Shigellae.
The importance of growth factors in the nutrition of shigellae was recognized by Koser and Saunders (1935) and it was shown that the growth factors present in tissue extract could be replaced by nicotinic acid (Koser et al., 1938). The importance of nicotinic acid and nicotinamide as growth factors in the nutrition of shigellae has been shown by Dorfman et al. (1939). Reports of other vitamins ...
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Haploid strains of S. cerevisiae were exposed to ultraviolet irradiation, and mutants were isolated by a modified layer plate procedure (Lederberg and Tatum, 1946), using a complete agar as the recovery layer. Six mutants were found which required adenine, two which required uracil. A seventh adenineless mutant was obtained through the courtesy of S. E. Reaume (Reaume and Tatum, 1949). Genetic ...
متن کاملThe mutagenic action of N-methyl-N'-nitro-N-nitrosoguanidine on Coprinus lagopus.
Optimal conditions, defined by the use of a known auxotrophic strain, are described for the mutagenesis of Coprinus Zagopus oidia by N-methyl-N’nitro-N-nitrosoguanidine (NTG), which is a very effective mutagen for bacteria and yeast. The oidia were best treated in phosphate buffer (PH 6.8) with a concentration of 15 pg. NTG/ml. for about 70 min. The yield of auxotrophs was considerably less tha...
متن کاملDark dependence of meiosis at elevated temperatures in the basidiomycete Coprinus lagopus.
Under continuous-light regimen Coprinus lagopus can enter and complete meiosis at 25 C, but is unable to do so at 35 C. The temperature-sensitive and the dark-dependent period is 10 hr before karyogamy. It is conjectured that the basidia are programmed for the initiation of meiosis during that 10-hr period.
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ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1967
ISSN: 0022-1287
DOI: 10.1099/00221287-47-2-163